When this is being sung in the Divine Liturgy of St. John Chrysostom, the faithful are about the receive the Body and Blood of our Lord and Saviour Jesus Christ. No instruments are used when chanting Byzantine music, only the human voice can be used and nothing else. Modern Greek American churches have adopted use of the organ, but the true Byzantine chant has no accompaniment.
"Let us who mystically represent the Cherubim,
and chant the thrice-holy hymn unto the life-creating Trinity.
Lay aside all earthly cares,
that we may receive the King of all,
escorted invisibly by the angelic host. Alleluia."